ABSTRACT
OBJECTIVES@#To detect the changes of microbial community functional diversity of corpses with different postmortem interval （PMI） and to evaluate forensic application value for estimating PMI.@*METHODS@#The cultivation of microbial community from the anal swabs of a Sus scrofa and a human corpse placed in field environment from 0 to 240 h after death was performed using the Biolog-Eco Microplate and the variations of the absorbance values were also monitored. Combined with the technology of forensic pathology and flies succession, the metabolic characteristics and changes of microbial community on the decomposed corpse under natural environment were also observed.@*RESULTS@#The diversity of microbial metabolism function was found to be negatively correlated with the number of maggots in the corpses. The freezing processing had the greatest impact on average well color development value at 0 h and the impact almost disappeared after 48 h. The diversity of microbial metabolism of the samples became relatively unstable after 192 h. The principal component analysis showed that 31 carbon sources could be consolidated for 5 principal components （accumulative contribution ratio >90%）.The carbon source tsquare-analysis showed that N-acetyl-D-glucosamine and L-serine were the dominant carbon sources for estimating the PMI （0=240 h） of the Sus scrofa and human corpse.@*CONCLUSIONS@#The Biolog-Eco method can be used to reveal the metabolic differences of the carbon resources utilization of the microbial community on the corpses during 0-240 h after death, which could provide a new basis for estimating the PMI.
Subject(s)
Humans , Biodiversity , Cadaver , Carbon , Postmortem Changes , Principal Component Analysis , Soil Microbiology , Time FactorsABSTRACT
OBJECTIVE@#To explore the growing development and community succession of main sarcosaphagous insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area and to estimate the postmortem interval (PMI).@*METHODS@#From early May to August in 2013, in Forensic Medical Examination Center of Shenzhen Public Security Bureau, the main insect species and the decomposition process were observed in two adult pig carcasses of simulative indoor and outdoor environment. The different decomposition stages and the community succession of insects were recorded.@*RESULTS@#The indoor and outdoor pig carcasses showed skeleton 412.5 and 325 hours after death, respectively. The main species of flies on pig carcasses were Chrysomya megacephala, Chrysomya rufifacies and Chrysomya chani. The main species of beetles were Crecphilus maxillosus, Necrobia ruficollis, Saprinus splendens and Dermestes maculatu. The dominant species of flies in the outdoor pig carcasses obviously produced the second generations due to the effect of mass rainfall, nor in the indoor pig carcasses.@*CONCLUSION@#There are regular patterns on the community succession of insects on pig carcasses in summer indoor and outdoor environment in Shenzhen area. The activity patterns of seven typical insects and their larva show important value for estimating PMI.
Subject(s)
Animals , Autopsy , Cadaver , China , Coleoptera , Death , Diptera , Environment , Insecta/growth & development , Larva , Population Dynamics , Postmortem Changes , Seasons , SwineABSTRACT
OBJECTIVE@#To explore the relationship between expression of endothelin in heart and ischemia.@*METHODS@#Using the RT-PCR method, we enquiry changes of ET-1 RNA after 60min ischemia in hearts of rats.@*RESULTS@#Although the two group appear positive, they are different significantly, which indicate that ischemia may lead to the increase of ET-1 mRNA.@*CONCLUSION@#The experiment, therefore, pave a way for immunochemical study.
Subject(s)
Animals , Female , Male , Rats , Endothelin-1/genetics , Image Processing, Computer-Assisted , Myocardial Ischemia/metabolism , Myocardium/pathology , RNA, Messenger/biosynthesis , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
OBJECTIVE@#To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.@*METHODS@#The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.@*RESULTS@#After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.@*CONCLUSION@#c-fos mRNA detection may become an important method for diagnosis of I/R.